TNF alpha ELISA Kit

Catalog No. ABIN5522445

Rat TNF alpha ELISA Kit Colorimetric assay for quantification of Rat TNF alpha and has been mentioned in 3+ publications.

Quick Overview for TNF alpha ELISA Kit (ABIN5522445)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.906 pg/mL - 250 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details TNF alpha ELISA Kit

Minimum Detection Limit: 3.906 pg/mL

Purpose: For quantitative detection in serum, plasma, tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificity: TNFα(Tumor Necrosis Factor Alpha)/TNF-α/DIF/TNF-alpha/TNFA/TNFSF2

Sensitivity: 2.344 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4 °C for 6 months

Expiry Date: 6 months

References for TNF alpha ELISA Kit (ABIN5522445)

Li, Deng, Shang, Wang, Xiao: "Blocking TRPA1 and TNF-α Signal Improves Bortezomib-Induced Neuropathic Pain." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 51, Issue 5, pp. 2098-2110, (2019) (PubMed).

Ranneh, Akim, Hamid, Khazaai, Fadel, Mahmoud: "Stingless bee honey protects against lipopolysaccharide induced-chronic subclinical systemic inflammation and oxidative stress by modulating Nrf2, NF-κB and p38 MAPK." in: Nutrition & metabolism, Vol. 16, pp. 15, (2019) (PubMed).

Bahtiar, Nurazizah, Roselina, Tambunan, Arsianti et al.: "Ethanolic extracts of babandotan leaves (Ageratum conyzoides L.) prevents inflammation and proteoglycan degradation by inhibiting TNF-α and MMP-9 on osteoarthritis rats induced by monosodium ..." in: Asian Pacific journal of tropical medicine, Vol. 10, Issue 3, pp. 270-277, (2017) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Background: TNFα(Tumor Necrosis Factor Alpha)/TNF-α/DIF/TNF-alpha/TNFA/TNFSF2

UniProt: P16599

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome